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| Salman, Doaa; Okuda, Liria Hiromi; Ueno, Akio; Dautu, George; Zhang, Feifei; Igarashi, Makoto. |
| Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA).... |
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Palavras-chave: T. gondii oocyst wall protein; Toxoplasma gondii. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4541 |
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| Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto. |
| Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in... |
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Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574 |
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| Pumidonming, Wilawan; Salman, Doaa; Gronsang, Dulyatad; Abdelbaset, E. Abdelbaset; Sangkaeo, Khamphon; Kawazu, Shin-ichiro; Igarashi, Makoto. |
| Gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. We investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower Northern Thailand and utilized molecular tools for species identification of hookworms and Opisthorchis viverrini. Fecal samples of 197 dogs and 180 cats were collected. Overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. Helminth infection found in both dogs and cats included hookworms, Spirometra spp., Taenia spp., Toxocara spp., 0. viverrini, Strongyloides spp. and Trichuris spp. Hookworms were the most common helminth in dogs, while Spirometra spp. were the most prevalent in cats. Among hookworm infection in dogs and... |
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Palavras-chave: Cat; Dog; Gastrointestinal helminth; Thailand; Zoonotic parasite. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4452 |
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